Optimal Production of Ganoderma formosanum Mycelium with Anti-Melanogenic Activity

Ganoderma formosanum is a medicinal mushroom endemic to Taiwan. The extraction of G. mycelium using ethyl acetate showed significant reduction melanin activity due the inhibition tyrosinase. To optimize production with anti-melanogenic activity, different variables (carbon sources and concentrations, initial pH value, temperature) were investigated. According results, optimal conditions included utilization 50 g/L lactose as carbon source at 7 25 °C for 9 days. Afterward, ethanolic extracts-optimized (GFE-O) was used study in vitro vivo. In B16-F10 melanoma cells treated GFE-O (0.1 mg/mL), intracellular content reduced 76% compared control group. By applying (0.05 mg/mL) vivo on zebrafish embryo, hypopigmentation observed, 62% no toxicological effects. results that inoculation can provide basis future large-scale maximize ingredients.